Zebrafish

Zebrafish

A Practical Guide to Husbandry, Welfare and Research Methodology

Edited by Claire Allen and Jean-Philippe Mocho
Hardback
October 2024
9781800629424
More details
  • Publisher
    CABI
  • Published
    7th October
  • ISBN 9781800629424
  • Language English
  • Pages 496 pp.
  • Size 6" x 9.75"
$200.00
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After securing popularity in home aquariums, zebrafish have also become an essential animal for biomedical research. Ensuring they are adequately cared for in research settings is therefore of the utmost importance and this book is designed to help laboratories develop good welfare and husbandry practices, refine procedural protocols and engage with ethical obligations.

A wide range of topics are addressed, starting with husbandry aspects such as environmental enrichment, nutrition, housing conditions, diseases, health monitoring, biosecurity and larval rearing. More experimental topics are then discussed, such as genotyping, ageing, imaging, behavioral studies, experimental design and statistics. Ethical challenges are also considered through the environmental impact of aquatic facilities and the 3Rs - Replacement, Reduction and Refinement - with a focus on anaesthesia, analgesia, euthanasia and severity assessment.

Zebrafish are a shoaling species by nature but this book also takes into account their individual needs by providing:

- practical advice from experienced zebrafish caretakers;

- bridges between data acquisition and fish welfare; and

- the latest guidance for reporting, reproducibility and compliance.

This book is written for zebrafish facility personnel and for researchers who are aware of the need for robust care for their zebrafish model. It also provides an unparalleled insight for ethics committee members with the intention to provide the best care possible for this ever-important fish.

The zebrafish model--Claire Allenand and Jean-Philippe Mocho

Environmental enrichment for zebrafish--Mollie E. Millington, Christian Lawrence, Lynne U. Sneddon and Claire Allen

Dietary requirements for zebrafish--Joana Monteiro, Sandra Martins, Rita Almeida, Carolina Cabrera and Ana Catarina Certal

Non-infectious diseases of zebrafish--Katrina N. Murray and Michael L. Kent

Infectious diseases of zebrafish--Joanna Cambray-Young

Adult zebrafish as a vertebrate model of ageing--Pam S. Ellis, Raquel R. Martins and Catarina M. Henriques

Organization of the zebrafish facility--Jean-Philippe Mocho

The 3Rs in zebrafish research--Kamar E. Ameen-Ali and Claire Allen

Statistical considerations for the breeding of zebrafish and their use in experiments--Amy Spencer, Peter Laud, Claire Allen, James Bull, Eleanor Stillman and Sara Hilditch

Genotyping methods in zebrafish--Claire Allen

Imaging of zebrafish--Karishma Chhabria

Behaviour in zebrafish--Raquel R. Martins and Paul G. Schroeder

Anaesthesia, analgesia and euthanasia of zebrafish--Jean-Philippe Mocho

Severity assignment for EU and UK procedures on Zebrafish--Jean-Philippe Mocho

Claire Allen

As a Named Animal Care and Welfare Office, specializing in zebrafish, Claire Allen now sits on several committees at the University of Sheffield concerned with the ethical review process. Claire is a frequent contributor and speaker at many national and international husbandry network meetings, conferences and events.

Jean-Philippe Mocho

After many years as a large animal vet, Jean-Philippe Mocho joined the Royal Veterinary College to dedicate his career to laboratory animals as a Named Veterinary Surgeon, providing his services to London's major academia and research institutes. His approach based on his experience of population medicine led him to the new shores of aquatic facilities.

fish for biomedical research; zebrafish; zebrafish welfare; zebrafish husbandry; fish welfare; fish husbandry; animal research ethics; fish nutrition; fish disease; fish health monitoring; fish biosecurity